Composite

Part:BBa_K4482011

Designed by: Matteo Da Ros   Group: iGEM22_NYC_Empire_State   (2022-09-30)


HIRMAb-HC-FGF2

The insulin receptor is a transmembrane protein expressed in many different tissues in the human body, including endothelial cells forming the blood vessels in the brain. The insulin receptor takes an active part in transporting insulin from the blood stream into the brain through the blood bran barrier (BBB). This property of the insulin receptor has been harnessed to for the active transport of therapeutic proteins through the BBB. The human insulin receptor monoclonal antibody (HIRMAb) was developed to hitchhike into the insulin receptor and be transported through the BBB to deliver cargoes into the brain when injected intravenously. Antibodies are immunoglobulins formed by two different peptides, a heavy chain and a light chain, that complex together in couples to form the mature antibody capable of recognizing its specific antigen. This part represents the coding sequence for the heavy chain of the HIRMAb recombinant antibody. The sequence was derived from the protein sequence registered in US patent US-8858950-B2. Fibroblast Growth Factor 2 is a protein that in humans is encoded by the FGF2 gene. The protein encoded by this gene, FGF-3, is a growth factor protein. According to literature, FGF-2 promotes differentiation and proliferation of neuronal stem cells. (Source: wikipedia). FGF-2 was fused to the C-terminus of HIRMAb-HC so that it could be transported from the blood stream through the BBB into the brain.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 262
    Illegal PstI site found at 1516
    Illegal PstI site found at 1522
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 262
    Illegal PstI site found at 1516
    Illegal PstI site found at 1522
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1342
    Illegal BamHI site found at 1413
    Illegal XhoI site found at 16
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 262
    Illegal PstI site found at 1516
    Illegal PstI site found at 1522
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 262
    Illegal PstI site found at 1516
    Illegal PstI site found at 1522
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1077
    Illegal BsaI.rc site found at 39
    Illegal SapI.rc site found at 387


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